Haynes Lab:Notebook/CRISPR Editing/2015/10/10: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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== | ==10/10/2015== | ||
Purified PCR product g031 EcoR1 donor | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''260''' | |||
| align="center" style="background:#f0f0f0;"|'''280''' | |||
| align="center" style="background:#f0f0f0;"|'''260/280''' | |||
| align="center" style="background:#f0f0f0;"|'''ng/µL''' | |||
|- | |||
| 0.064||0.035||1.825||63.731 | |||
|} | |||
Luciferase assay of gal4 with puro or dox added at two times, 10/6 and 10/1. Dox added earlier didn't show an increase in silencing. | |||
<br><br> | |||
Transfected gal4-eed open and closed chromatin with | |||
:750ng of g031 + 250ng of g031 EcoRI donor | |||
:750ng of g034 + 250ng of g034 EcoRI donor<br> | |||
antibiotic free media | |||
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Latest revision as of 01:14, 27 September 2017
Project name | Main project page Previous entry Next entry | ||||||||
10/10/2015Purified PCR product g031 EcoR1 donor
Luciferase assay of gal4 with puro or dox added at two times, 10/6 and 10/1. Dox added earlier didn't show an increase in silencing.
antibiotic free media |