Haynes Lab:Notebook/CRISPR Editing/2015/10/07: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
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==03//2015==
==10/07/2015==


PCR purification
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Cell type'''
| align="center" style="background:#f0f0f0;"|'''drug'''
| align="center" style="background:#f0f0f0;"|'''siRNA'''
| align="center" style="background:#f0f0f0;"|'''drug'''
| align="center" style="background:#f0f0f0;"|'''gRNA'''
| align="center" style="background:#f0f0f0;"|'''rep'''
| align="center" style="background:#f0f0f0;"|'''260'''
| align="center" style="background:#f0f0f0;"|'''280'''
| align="center" style="background:#f0f0f0;"|'''260/280'''
| align="center" style="background:#f0f0f0;"|'''ng/µL'''
| align="center" style="background:#f0f0f0;"|'''ul for 400ng'''
| align="center" style="background:#f0f0f0;"|'''ul ann buff'''
| align="center" style="background:#f0f0f0;"|'''ul water'''
|-
| Gal4-EED||dox||EZH2||puro||g025||1||0.104||0.057||1.834||104.399||3.83||1.50||9.67
|-
| Gal4-EED||dox||EZH2||puro||g025||2||0.087||0.046||1.876||86.528||4.62||1.50||8.88
|-
| Gal4-EED||dox||EZH2||puro||g025||3||0.096||0.052||1.821||95.567||4.19||1.50||9.31
|-
| Gal4-EED||dox||none||puro||g025||1||0.117||0.063||1.863||116.936||3.42||1.50||10.08
|-
| Gal4-EED||dox||none||puro||g025||2||0.077||0.043||1.813||77.314||5.17||1.50||8.33
|-
| Gal4-EED||dox||none||puro||g025||3||0.088||0.046||1.901||88.068||4.54||1.50||8.96
|}


{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Cell type'''
| align="center" style="background:#f0f0f0;"|'''drug'''
| align="center" style="background:#f0f0f0;"|'''siRNA'''
| align="center" style="background:#f0f0f0;"|'''drug'''
| align="center" style="background:#f0f0f0;"|'''gRNA'''
| align="center" style="background:#f0f0f0;"|'''rep'''
| align="center" style="background:#f0f0f0;"|'''260'''
| align="center" style="background:#f0f0f0;"|'''280'''
| align="center" style="background:#f0f0f0;"|'''260/280'''
| align="center" style="background:#f0f0f0;"|'''ng/µL'''
| align="center" style="background:#f0f0f0;"|'''ul for 400ng'''
| align="center" style="background:#f0f0f0;"|'''ul ann buff'''
| align="center" style="background:#f0f0f0;"|'''ul water'''
|-
| Gal4-EED||dox||EZH2||puro||g034||1||0.054||0.031||1.755||54.126||7.39||1.50||6.11
|-
| Gal4-EED||dox||EZH2||puro||g034||2||0.063||0.035||1.829||63.131||6.34||1.50||7.16
|-
| Gal4-EED||dox||EZH2||puro||g034||3||0.054||0.032||1.706||54.267||7.37||1.50||6.13
|-
| Gal4-EED||dox||none||puro||g034||1||0.068||0.038||1.81||68.014||5.88||1.50||7.62
|-
| Gal4-EED||dox||none||puro||g034||2||0.052||0.029||1.811||52.032||7.69||1.50||5.81
|-
| Gal4-EED||dox||none||puro||g034||3||0.05||0.028||1.777||50.273||7.96||1.50||5.54
|}
g034 EcoRI donor
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''260'''
| align="center" style="background:#f0f0f0;"|'''280'''
| align="center" style="background:#f0f0f0;"|'''260/280'''
| align="center" style="background:#f0f0f0;"|'''ng/µL'''
|-
| 0.132||0.071||1.864||132.19
|}


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Latest revision as of 01:14, 27 September 2017

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10/07/2015

PCR purification

Cell type drug siRNA drug gRNA rep 260 280 260/280 ng/µL ul for 400ng ul ann buff ul water
Gal4-EED dox EZH2 puro g025 1 0.104 0.057 1.834 104.399 3.83 1.50 9.67
Gal4-EED dox EZH2 puro g025 2 0.087 0.046 1.876 86.528 4.62 1.50 8.88
Gal4-EED dox EZH2 puro g025 3 0.096 0.052 1.821 95.567 4.19 1.50 9.31
Gal4-EED dox none puro g025 1 0.117 0.063 1.863 116.936 3.42 1.50 10.08
Gal4-EED dox none puro g025 2 0.077 0.043 1.813 77.314 5.17 1.50 8.33
Gal4-EED dox none puro g025 3 0.088 0.046 1.901 88.068 4.54 1.50 8.96
Cell type drug siRNA drug gRNA rep 260 280 260/280 ng/µL ul for 400ng ul ann buff ul water
Gal4-EED dox EZH2 puro g034 1 0.054 0.031 1.755 54.126 7.39 1.50 6.11
Gal4-EED dox EZH2 puro g034 2 0.063 0.035 1.829 63.131 6.34 1.50 7.16
Gal4-EED dox EZH2 puro g034 3 0.054 0.032 1.706 54.267 7.37 1.50 6.13
Gal4-EED dox none puro g034 1 0.068 0.038 1.81 68.014 5.88 1.50 7.62
Gal4-EED dox none puro g034 2 0.052 0.029 1.811 52.032 7.69 1.50 5.81
Gal4-EED dox none puro g034 3 0.05 0.028 1.777 50.273 7.96 1.50 5.54

g034 EcoRI donor

260 280 260/280 ng/µL
0.132 0.071 1.864 132.19


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