Haynes Lab:Notebook/CRISPR Editing/2015/10/06: Difference between revisions

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Col pur Gal4 puro/dox g055 samples, set up annealing reaction
Col pur Gal4 puro/dox g055 samples, set up annealing reaction
 
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Cell type'''
| align="center" style="background:#f0f0f0;"|'''drug'''
| align="center" style="background:#f0f0f0;"|'''gRNA'''
| align="center" style="background:#f0f0f0;"|'''rep'''
| align="center" style="background:#f0f0f0;"|'''260'''
| align="center" style="background:#f0f0f0;"|'''280'''
| align="center" style="background:#f0f0f0;"|'''260/280'''
| align="center" style="background:#f0f0f0;"|'''ng/µL'''
| align="center" style="background:#f0f0f0;"|'''ul for 400'''
| align="center" style="background:#f0f0f0;"|'''ul ann buff'''
| align="center" style="background:#f0f0f0;"|'''ul water'''
|-
| Gal4-EED||puro||g055||1||0.068||0.035||1.93||68.229||5.86||1.50||7.64
|-
| Gal4-EED||puro||g055||2||0.072||0.038||1.87||71.618||5.59||1.50||7.91
|-
| Gal4-EED||puro||g055||3||0.079||0.042||1.882||78.826||5.07||1.50||8.43
|-
| Gal4-EED||dox||g055||1||0.07||0.038||1.839||69.741||5.74||1.50||7.76
|-
| Gal4-EED||dox||g055||2||0.074||0.039||1.875||73.714||5.43||1.50||8.07
|-
| Gal4-EED||dox||g055||3||0.074||0.042||1.773||74.442||5.37||1.50||8.13
|}


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Revision as of 08:50, 6 October 2015

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10/06/2015

Ran PCR of Gal4 siRNA g025 g034 samples with P197/198 on gel



Col pur Gal4 puro/dox g054 samples, set up annealing reaction

Cell type drug gRNA rep 260 280 260/280 ng/µL ul for 400 ul ann buff ul water
Gal4-EED puro g054 1 0.1 0.054 1.87 100.189 3.99 1.50 9.51
Gal4-EED puro g054 2 0.09 0.047 1.91 90.466 4.42 1.50 9.08
Gal4-EED puro g054 3 0.105 0.057 1.862 105.5 3.79 1.50 9.71
Gal4-EED dox g054 1 0.104 0.054 1.919 103.727 3.86 1.50 9.64
Gal4-EED dox g054 2 0.115 0.063 1.825 114.985 3.48 1.50 10.02
Gal4-EED dox g054 3 0.092 0.049 1.891 92.038 4.35 1.50 9.15



Col pur Gal4 puro/dox g055 samples, set up annealing reaction

Cell type drug gRNA rep 260 280 260/280 ng/µL ul for 400 ul ann buff ul water
Gal4-EED puro g055 1 0.068 0.035 1.93 68.229 5.86 1.50 7.64
Gal4-EED puro g055 2 0.072 0.038 1.87 71.618 5.59 1.50 7.91
Gal4-EED puro g055 3 0.079 0.042 1.882 78.826 5.07 1.50 8.43
Gal4-EED dox g055 1 0.07 0.038 1.839 69.741 5.74 1.50 7.76
Gal4-EED dox g055 2 0.074 0.039 1.875 73.714 5.43 1.50 8.07
Gal4-EED dox g055 3 0.074 0.042 1.773 74.442 5.37 1.50 8.13


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