Haynes Lab:Notebook/CRISPR Editing/2015/10/06: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
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==03//2015==
==10/06/2015==
 
Ran PCR of Gal4 siRNA g025 g034 samples with P197/198 on gel
 
:no bands in neg ctrl, all other lanes look great
<br><br>
Col pur Gal4 puro/dox g054 samples, set up annealing reaction
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Cell type'''
| align="center" style="background:#f0f0f0;"|'''drug'''
| align="center" style="background:#f0f0f0;"|'''gRNA'''
| align="center" style="background:#f0f0f0;"|'''rep'''
| align="center" style="background:#f0f0f0;"|'''260'''
| align="center" style="background:#f0f0f0;"|'''280'''
| align="center" style="background:#f0f0f0;"|'''260/280'''
| align="center" style="background:#f0f0f0;"|'''ng/µL'''
| align="center" style="background:#f0f0f0;"|'''ul for 400'''
| align="center" style="background:#f0f0f0;"|'''ul ann buff'''
| align="center" style="background:#f0f0f0;"|'''ul water'''
|-
| Gal4-EED||puro||g054||1||0.1||0.054||1.87||100.189||3.99||1.50||9.51
|-
| Gal4-EED||puro||g054||2||0.09||0.047||1.91||90.466||4.42||1.50||9.08
|-
| Gal4-EED||puro||g054||3||0.105||0.057||1.862||105.5||3.79||1.50||9.71
|-
| Gal4-EED||dox||g054||1||0.104||0.054||1.919||103.727||3.86||1.50||9.64
|-
| Gal4-EED||dox||g054||2||0.115||0.063||1.825||114.985||3.48||1.50||10.02
|-
| Gal4-EED||dox||g054||3||0.092||0.049||1.891||92.038||4.35||1.50||9.15
|}
<br><br>
Col pur Gal4 puro/dox g055 samples, set up annealing reaction
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Cell type'''
| align="center" style="background:#f0f0f0;"|'''drug'''
| align="center" style="background:#f0f0f0;"|'''gRNA'''
| align="center" style="background:#f0f0f0;"|'''rep'''
| align="center" style="background:#f0f0f0;"|'''260'''
| align="center" style="background:#f0f0f0;"|'''280'''
| align="center" style="background:#f0f0f0;"|'''260/280'''
| align="center" style="background:#f0f0f0;"|'''ng/µL'''
| align="center" style="background:#f0f0f0;"|'''ul for 400'''
| align="center" style="background:#f0f0f0;"|'''ul ann buff'''
| align="center" style="background:#f0f0f0;"|'''ul water'''
|-
| Gal4-EED||puro||g055||1||0.068||0.035||1.93||68.229||5.86||1.50||7.64
|-
| Gal4-EED||puro||g055||2||0.072||0.038||1.87||71.618||5.59||1.50||7.91
|-
| Gal4-EED||puro||g055||3||0.079||0.042||1.882||78.826||5.07||1.50||8.43
|-
| Gal4-EED||dox||g055||1||0.07||0.038||1.839||69.741||5.74||1.50||7.76
|-
| Gal4-EED||dox||g055||2||0.074||0.039||1.875||73.714||5.43||1.50||8.07
|-
| Gal4-EED||dox||g055||3||0.074||0.042||1.773||74.442||5.37||1.50||8.13
|}


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Latest revision as of 01:14, 27 September 2017

Project name Main project page
Previous entry      Next entry

10/06/2015

Ran PCR of Gal4 siRNA g025 g034 samples with P197/198 on gel

no bands in neg ctrl, all other lanes look great



Col pur Gal4 puro/dox g054 samples, set up annealing reaction

Cell type drug gRNA rep 260 280 260/280 ng/µL ul for 400 ul ann buff ul water
Gal4-EED puro g054 1 0.1 0.054 1.87 100.189 3.99 1.50 9.51
Gal4-EED puro g054 2 0.09 0.047 1.91 90.466 4.42 1.50 9.08
Gal4-EED puro g054 3 0.105 0.057 1.862 105.5 3.79 1.50 9.71
Gal4-EED dox g054 1 0.104 0.054 1.919 103.727 3.86 1.50 9.64
Gal4-EED dox g054 2 0.115 0.063 1.825 114.985 3.48 1.50 10.02
Gal4-EED dox g054 3 0.092 0.049 1.891 92.038 4.35 1.50 9.15



Col pur Gal4 puro/dox g055 samples, set up annealing reaction

Cell type drug gRNA rep 260 280 260/280 ng/µL ul for 400 ul ann buff ul water
Gal4-EED puro g055 1 0.068 0.035 1.93 68.229 5.86 1.50 7.64
Gal4-EED puro g055 2 0.072 0.038 1.87 71.618 5.59 1.50 7.91
Gal4-EED puro g055 3 0.079 0.042 1.882 78.826 5.07 1.50 8.43
Gal4-EED dox g055 1 0.07 0.038 1.839 69.741 5.74 1.50 7.76
Gal4-EED dox g055 2 0.074 0.039 1.875 73.714 5.43 1.50 8.07
Gal4-EED dox g055 3 0.074 0.042 1.773 74.442 5.37 1.50 8.13


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