Haynes Lab:Notebook/CRISPR Editing/2015/10/02: Difference between revisions
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| Gal4-EED||Dox||g046||3||0.071||0.035||2||70.905||5.64||1.50||7.86 | | Gal4-EED||Dox||g046||3||0.071||0.035||2||70.905||5.64||1.50||7.86 | ||
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Set up g032 for surveyor, 1ul surveyor, 1ul enhancer, 60mins at 42degC, add stop solution, dilute 1:20, run on BA | |||
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Ran g054, g055 nested PCR on gel. g054 looked good, a faint band in the negative control lane. g055 was faint, no band in negative control lane. | |||
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Isolated gDNA from siRNA samples. EZH2 and blank transfection, then either g025 or g034, in triplicate. | |||
Revision as of 17:13, 2 October 2015
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10/02/2015Spec'd and set up annealing reaction for g032 and g046 +/- chromatin
Set up g032 for surveyor, 1ul surveyor, 1ul enhancer, 60mins at 42degC, add stop solution, dilute 1:20, run on BA
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