Haynes Lab:Notebook/CRISPR Editing/2015/10/02: Difference between revisions

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(Autocreate 2015/10/02 Entry for Haynes_Lab:Notebook/CRISPR_Editing)
 
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==03//2015==
==10/02/2015==
 
Spec'd and set up annealing reaction for g032 and g046 +/- chromatin
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Cell type'''
| align="center" style="background:#f0f0f0;"|'''Drug'''
| align="center" style="background:#f0f0f0;"|'''gRNA'''
| align="center" style="background:#f0f0f0;"|'''rep'''
| align="center" style="background:#f0f0f0;"|'''260'''
| align="center" style="background:#f0f0f0;"|'''280'''
| align="center" style="background:#f0f0f0;"|'''260/280'''
| align="center" style="background:#f0f0f0;"|'''ng/µL'''
| align="center" style="background:#f0f0f0;"|'''ul for 400ng'''
| align="center" style="background:#f0f0f0;"|'''ul ann buff'''
| align="center" style="background:#f0f0f0;"|'''ul H20'''
|-
| Gal4-EED||Puro||g032||1||0.102||0.052||1.953||102.256||3.91||1.50||9.59
|-
| Gal4-EED||Puro||g032||2||0.093||0.05||1.873||93.185||4.29||1.50||9.21
|-
| Gal4-EED||Puro||g032||3||0.069||0.036||1.907||69.418||5.76||1.50||7.74
|-
| Gal4-EED||Dox||g032||1||0.083||0.043||1.941||83.246||4.81||1.50||8.69
|-
| Gal4-EED||Dox||g032||2||0.087||0.045||1.912||86.929||4.60||1.50||8.90
|-
| Gal4-EED||Dox||g032||3||0.092||0.049||1.902||92.435||4.33||1.50||9.17
|-
| Gal4-EED||Puro||g046||1||0.081||0.042||1.913||80.615||4.96||1.50||8.54
|-
| Gal4-EED||Puro||g046||2||0.076||0.041||1.834||75.671||5.29||1.50||8.21
|-
| Gal4-EED||Puro||g046||3||0.077||0.04||1.927||76.588||5.22||1.50||8.28
|-
| Gal4-EED||Dox||g046||1||0.063||0.034||1.864||63.022||6.35||1.50||7.15
|-
| Gal4-EED||Dox||g046||2||0.081||0.042||1.909||80.568||4.96||1.50||8.54
|-
| Gal4-EED||Dox||g046||3||0.071||0.035||2||70.905||5.64||1.50||7.86
|}




Revision as of 12:52, 2 October 2015

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10/02/2015

Spec'd and set up annealing reaction for g032 and g046 +/- chromatin

Cell type Drug gRNA rep 260 280 260/280 ng/µL ul for 400ng ul ann buff ul H20
Gal4-EED Puro g032 1 0.102 0.052 1.953 102.256 3.91 1.50 9.59
Gal4-EED Puro g032 2 0.093 0.05 1.873 93.185 4.29 1.50 9.21
Gal4-EED Puro g032 3 0.069 0.036 1.907 69.418 5.76 1.50 7.74
Gal4-EED Dox g032 1 0.083 0.043 1.941 83.246 4.81 1.50 8.69
Gal4-EED Dox g032 2 0.087 0.045 1.912 86.929 4.60 1.50 8.90
Gal4-EED Dox g032 3 0.092 0.049 1.902 92.435 4.33 1.50 9.17
Gal4-EED Puro g046 1 0.081 0.042 1.913 80.615 4.96 1.50 8.54
Gal4-EED Puro g046 2 0.076 0.041 1.834 75.671 5.29 1.50 8.21
Gal4-EED Puro g046 3 0.077 0.04 1.927 76.588 5.22 1.50 8.28
Gal4-EED Dox g046 1 0.063 0.034 1.864 63.022 6.35 1.50 7.15
Gal4-EED Dox g046 2 0.081 0.042 1.909 80.568 4.96 1.50 8.54
Gal4-EED Dox g046 3 0.071 0.035 2 70.905 5.64 1.50 7.86



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