Haynes Lab:Notebook/CRISPR Editing/2015/10/02

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10/02/2015

Spec'd and set up annealing reaction for g032 and g046 +/- chromatin

Cell type Drug gRNA rep 260 280 260/280 ng/µL ul for 400ng ul ann buff ul H20
Gal4-EED Puro g032 1 0.102 0.052 1.953 102.256 3.91 1.50 9.59
Gal4-EED Puro g032 2 0.093 0.05 1.873 93.185 4.29 1.50 9.21
Gal4-EED Puro g032 3 0.069 0.036 1.907 69.418 5.76 1.50 7.74
Gal4-EED Dox g032 1 0.083 0.043 1.941 83.246 4.81 1.50 8.69
Gal4-EED Dox g032 2 0.087 0.045 1.912 86.929 4.60 1.50 8.90
Gal4-EED Dox g032 3 0.092 0.049 1.902 92.435 4.33 1.50 9.17
Gal4-EED Puro g046 1 0.081 0.042 1.913 80.615 4.96 1.50 8.54
Gal4-EED Puro g046 2 0.076 0.041 1.834 75.671 5.29 1.50 8.21
Gal4-EED Puro g046 3 0.077 0.04 1.927 76.588 5.22 1.50 8.28
Gal4-EED Dox g046 1 0.063 0.034 1.864 63.022 6.35 1.50 7.15
Gal4-EED Dox g046 2 0.081 0.042 1.909 80.568 4.96 1.50 8.54
Gal4-EED Dox g046 3 0.071 0.035 2 70.905 5.64 1.50 7.86



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