Haynes Lab:Notebook/CRISPR Editing/2015/09/30: Difference between revisions

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(Autocreate 2015/09/30 Entry for Haynes_Lab:Notebook/CRISPR_Editing)
 
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==03//2015==
==09/30/2015==
 
Nested PCR of g032 samples. Dilute each P197/198 reaction 1:1000. PCR with P149/174.<br>
Nested PCR of g046 samples. Dilute each P197/198 reaction 1:1000. PCR with P153/160.<br>
Ran on gel, no bands in negative ctrl lanes<br>
PCR purified<br><br>


Nested PCR of g054 samples. Dilute each P197/198 reaction 1:1000. PCR with P149/162.<br>
Nested PCR of g055 samples. Dilute each P197/198 reaction 1:1000. PCR with P153/160.<br>


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Revision as of 17:30, 2 October 2015

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09/30/2015

Nested PCR of g032 samples. Dilute each P197/198 reaction 1:1000. PCR with P149/174.
Nested PCR of g046 samples. Dilute each P197/198 reaction 1:1000. PCR with P153/160.
Ran on gel, no bands in negative ctrl lanes
PCR purified

Nested PCR of g054 samples. Dilute each P197/198 reaction 1:1000. PCR with P149/162.
Nested PCR of g055 samples. Dilute each P197/198 reaction 1:1000. PCR with P153/160.