Haynes Lab:Notebook/CRISPR Editing/2015/09/29: Difference between revisions

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isolated gDNA from puro dox g054 g055 cells from a few weeks ago. Set up PCR with P197/198
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Cell type'''
| align="center" style="background:#f0f0f0;"|'''drug'''
| align="center" style="background:#f0f0f0;"|'''gRNA'''
| align="center" style="background:#f0f0f0;"|'''rep'''
| align="center" style="background:#f0f0f0;"|'''260'''
| align="center" style="background:#f0f0f0;"|'''280'''
| align="center" style="background:#f0f0f0;"|'''260/280'''
| align="center" style="background:#f0f0f0;"|'''ng/µL'''
| align="center" style="background:#f0f0f0;"|'''ul for 200ng'''
| align="center" style="background:#f0f0f0;"|'''ul water to 10ul'''
|-
| Gal4-EED||puro||g054||1||0.033||0.014||2.289||32.879||6.08||3.92
|-
| Gal4-EED||puro||g054||2||0.026||0.012||2.232||26.294||7.61||2.39
|-
| Gal4-EED||puro||g054||3||0.028||0.013||2.252||28.36||7.05||2.95
|-
| Gal4-EED||dox||g054||1||0.069||0.033||2.102||69.023||2.90||7.10
|-
| Gal4-EED||dox||g054||2||0.07||0.034||2.091||70.196||2.85||7.15
|-
| Gal4-EED||dox||g054||3||0.061||0.03||2.052||61.451||3.25||6.75
|-
| Gal4-EED||puro||g055||1||0.166||0.082||2.033||166.248||1.20||8.80
|-
| Gal4-EED||puro||g055||2||0.225||0.114||1.981||224.964||0.89||9.11
|-
| Gal4-EED||puro||g055||3||0.19||0.093||2.039||190.432||1.05||8.95
|-
| Gal4-EED||dox||g055||1||0.1||0.05||2.021||100.183||2.00||8.00
|-
| Gal4-EED||dox||g055||2||0.115||0.055||2.079||114.683||1.74||8.26
|-
| Gal4-EED||dox||g055||3||0.117||0.057||2.07||117.461||1.70||8.30
|}


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Revision as of 12:14, 29 September 2015

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03//2015

isolated gDNA from puro dox g032 g046 cells from a few weeks ago. Set up PCR with P197/198

Cell type drug gRNA rep 260 280 260/280 ng/µL ul for 200ng 5ul - 200ng
Gal4-EED puro g032 1 0.232 0.115 2.012 231.855 0.86 4.14
Gal4-EED puro g032 2 0.218 0.108 2.02 217.954 0.92 4.08
Gal4-EED puro g032 3 0.178 0.088 2.008 177.513 1.13 3.87
Gal4-EED dox g032 1 0.104 0.053 1.974 104.245 1.92 3.08
Gal4-EED dox g032 2 0.073 0.04 1.84 72.906 2.74 2.26
Gal4-EED dox g032 3 0.086 0.043 2.007 85.616 2.34 2.66
Gal4-EED puro g046 1 0.248 0.124 1.994 247.506 0.81 4.19
Gal4-EED puro g046 2 0.302 0.149 2.027 302.468 0.66 4.34
Gal4-EED puro g046 3 0.289 0.146 1.985 289.425 0.69 4.31
Gal4-EED dox g046 1 0.067 0.035 1.911 66.504 3.01 1.99
Gal4-EED dox g046 2 0.104 0.054 1.937 104.022 1.92 3.08
Gal4-EED dox g046 3 0.074 0.036 2.023 73.845 2.71 2.29

isolated gDNA from puro dox g054 g055 cells from a few weeks ago. Set up PCR with P197/198

Cell type drug gRNA rep 260 280 260/280 ng/µL ul for 200ng ul water to 10ul
Gal4-EED puro g054 1 0.033 0.014 2.289 32.879 6.08 3.92
Gal4-EED puro g054 2 0.026 0.012 2.232 26.294 7.61 2.39
Gal4-EED puro g054 3 0.028 0.013 2.252 28.36 7.05 2.95
Gal4-EED dox g054 1 0.069 0.033 2.102 69.023 2.90 7.10
Gal4-EED dox g054 2 0.07 0.034 2.091 70.196 2.85 7.15
Gal4-EED dox g054 3 0.061 0.03 2.052 61.451 3.25 6.75
Gal4-EED puro g055 1 0.166 0.082 2.033 166.248 1.20 8.80
Gal4-EED puro g055 2 0.225 0.114 1.981 224.964 0.89 9.11
Gal4-EED puro g055 3 0.19 0.093 2.039 190.432 1.05 8.95
Gal4-EED dox g055 1 0.1 0.05 2.021 100.183 2.00 8.00
Gal4-EED dox g055 2 0.115 0.055 2.079 114.683 1.74 8.26
Gal4-EED dox g055 3 0.117 0.057 2.07 117.461 1.70 8.30


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