Haynes Lab:Notebook/CRISPR Editing/2015/09/04: Difference between revisions
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==09/04/2015== | ==09/04/2015== | ||
Cells in 23 well plates were well spaced, probably about 70% confluent but proceeded with the transfections anyway. Did g025, g031, g044, and g054. Removed media before and added back media without antibiotics. | |||
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| align="center" style="background:#f0f0f0;"|'''Step 1: Making .5ug/10ul plasmid stocks''' | | align="center" style="background:#f0f0f0;"|'''Step 1: Making .5ug/10ul plasmid stocks''' | ||
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Removed media from gal4-eed cells that had been under dox for 96 hours. washed with PBS and replaced with media with puro. washed and replaced media on the puro cells as well. will use these for siRNA transfections. | |||
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Latest revision as of 01:08, 27 September 2017
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09/04/2015Cells in 23 well plates were well spaced, probably about 70% confluent but proceeded with the transfections anyway. Did g025, g031, g044, and g054. Removed media before and added back media without antibiotics.
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