Haynes Lab:Notebook/CRISPR Editing/2015/08/31: Difference between revisions

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(Autocreate 2015/08/31 Entry for Haynes_Lab:Notebook/CRISPR_Editing)
 
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==03//2015==
==08/31/2015==
 
Surveyor assay on Luc14 dilutions in triplicate, 1ug, 0.1ug, and 0.01ug g034. 1ul surveyor, 1ul enhancer, 1hour at 42deg. after 1h, added 1.9ul of stop solution, diluted 1:20 in water, ran on BA.
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PCR purified the samples from the dilution experiment that didn't have enough DNA.
<br><br>





Revision as of 11:06, 31 August 2015

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08/31/2015

Surveyor assay on Luc14 dilutions in triplicate, 1ug, 0.1ug, and 0.01ug g034. 1ul surveyor, 1ul enhancer, 1hour at 42deg. after 1h, added 1.9ul of stop solution, diluted 1:20 in water, ran on BA.

PCR purified the samples from the dilution experiment that didn't have enough DNA.