Haynes Lab:Notebook/CRISPR Editing/2015/08/31: Difference between revisions
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Rene M Davis (talk | contribs) (Autocreate 2015/08/31 Entry for Haynes_Lab:Notebook/CRISPR_Editing) |
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== | ==08/31/2015== | ||
Surveyor assay on Luc14 dilutions in triplicate, 1ug, 0.1ug, and 0.01ug g034. 1ul surveyor, 1ul enhancer, 1hour at 42deg. after 1h, added 1.9ul of stop solution, diluted 1:20 in water, ran on BA. | |||
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PCR purified the samples from the dilution experiment that didn't have enough DNA. | |||
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Revision as of 11:06, 31 August 2015
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08/31/2015Surveyor assay on Luc14 dilutions in triplicate, 1ug, 0.1ug, and 0.01ug g034. 1ul surveyor, 1ul enhancer, 1hour at 42deg. after 1h, added 1.9ul of stop solution, diluted 1:20 in water, ran on BA.
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