Haynes Lab:Notebook/CRISPR Editing/2015/08/27: Difference between revisions

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| align="center" style="background:#f0f0f0;"|'''260/280'''
| align="center" style="background:#f0f0f0;"|'''260/280'''
| align="center" style="background:#f0f0f0;"|'''ng/µL'''
| align="center" style="background:#f0f0f0;"|'''ng/µL'''
| align="center" style="background:#f0f0f0;"|'''ng/µL'''
| align="center" style="background:#f0f0f0;"|'''average'''
| align="center" style="background:#f0f0f0;"|'''ul for 400ng'''
| align="center" style="background:#f0f0f0;"|'''ul for 400ng'''
| align="center" style="background:#f0f0f0;"|'''ul ann buff'''
| align="center" style="background:#f0f0f0;"|'''ul ann buff'''
| align="center" style="background:#f0f0f0;"|'''ul H2O'''
| align="center" style="background:#f0f0f0;"|'''ul H2O'''
|-
|-
| Luc14||g030||0.096||0.052||1.855||95.552||4.19||1.5||9.31
| Luc14||g030||0.073||0.039||1.889||76.357||73.096||74.73||5.35||1.50||8.15
|-
|-
| Luc14||g033||0.049||0.027||1.802||48.747||8.21||1.5||5.29
| Luc14||g033||0.043||0.023||1.889||43.492||43.386||43.44||9.21||1.50||4.29
|-
|-
| Luc14||g036||0.057||0.03||1.9||56.608||7.07||1.5||6.43
| Luc14||g036||0.084||0.045||1.837||80.736||83.56||82.15||4.87||1.50||8.63
|-
|-
| Luc14||g040||0.067||0.035||1.927||66.681||6.00||1.5||7.50
| Luc14||g040||0.082||0.044||1.88||82.825||82.088||82.46||4.85||1.50||8.65
|-
|-
| Luc14||g040||0.054||0.029||1.848||53.968||7.41||1.5||6.09
| Luc14||g040||0.065||0.036||1.794||63.731||64.87||64.30||6.22||1.50||7.28
|-
|-
| Luc14||g041||0.072||0.038||1.864||71.533||5.59||1.5||7.91
| Luc14||g041||0.055||0.029||1.888||53.011||54.684||53.85||7.43||1.50||6.07
|-
|-
| Luc14||g042||0.042||0.023||1.865||42.202||9.48||1.5||4.02
| Luc14||g042||0.045||0.024||1.885||47.205||45.157||46.18||8.66||1.50||4.84
|-
|-
| Luc14||g043||0.082||0.045||1.793||81.554||4.90||1.5||8.60
| Luc14||g043||0.044||0.023||1.88||45.143||43.898||44.52||8.98||1.50||4.52
|}
|}


Revision as of 12:58, 27 August 2015

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08/27/2015

Set up annealing reaction for Surveyor of Luc14 dilution of g034 treated cells collected 7/8/15.

Cell type drug ug g034 rep ul for 400ng ul buffer ul water for 15ul
Luc14 - 1 1 6.13 1.50 7.37
Luc14 - 1 2 6.03 1.50 7.47
Luc14 - 1 3 6.11 1.50 7.39
Luc14 - 0.1 1 5.98 1.50 7.52
Luc14 - 0.1 2 6.17 1.50 7.33
Luc14 - 0.1 3 6.60 1.50 6.90
Luc14 - 0.01 1 5.87 1.50 7.63
Luc14 - 0.01 2 6.08 1.50 7.42
Luc14 - 0.01 3 6.87 1.50 6.63


Some PCR purifications from that same dilution experiment didn't have high concentrations so redid PCR with P163/215

Gal4-EED + dox 0.1ul g034 rep 3
Gal4-EED + dox 0.01ul g034 rep 1



Set up annealing reaction for Surveyor of Luc14 singlet from initial screen of gRNAs around the end of 2014

Luc14 g029 260 280 260/280 ng/µL ng/µL average ul for 400ng ul ann buff ul H2O
Luc14 g030 0.073 0.039 1.889 76.357 73.096 74.73 5.35 1.50 8.15
Luc14 g033 0.043 0.023 1.889 43.492 43.386 43.44 9.21 1.50 4.29
Luc14 g036 0.084 0.045 1.837 80.736 83.56 82.15 4.87 1.50 8.63
Luc14 g040 0.082 0.044 1.88 82.825 82.088 82.46 4.85 1.50 8.65
Luc14 g040 0.065 0.036 1.794 63.731 64.87 64.30 6.22 1.50 7.28
Luc14 g041 0.055 0.029 1.888 53.011 54.684 53.85 7.43 1.50 6.07
Luc14 g042 0.045 0.024 1.885 47.205 45.157 46.18 8.66 1.50 4.84
Luc14 g043 0.044 0.023 1.88 45.143 43.898 44.52 8.98 1.50 4.52



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