Haynes Lab:Notebook/CRISPR Editing/2015/08/27: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
==08/27/2015== | ==08/27/2015== | ||
Collected g034 transfections at 48 hours. Flow and gDNA extraction, three wells for g4+p and three wells for g4+d | |||
<br><br> | |||
Set up annealing reaction for Surveyor of Luc14 dilution of g034 treated cells collected 7/8/15. | Set up annealing reaction for Surveyor of Luc14 dilution of g034 treated cells collected 7/8/15. | ||
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| align="center" style="background:#f0f0f0;"|'''Luc14''' | | align="center" style="background:#f0f0f0;"|'''Luc14''' | ||
| align="center" style="background:#f0f0f0;"|''' | | align="center" style="background:#f0f0f0;"|'''gRNA''' | ||
| align="center" style="background:#f0f0f0;"|'''260''' | | align="center" style="background:#f0f0f0;"|'''260''' | ||
| align="center" style="background:#f0f0f0;"|'''280''' | | align="center" style="background:#f0f0f0;"|'''280''' | ||
| align="center" style="background:#f0f0f0;"|'''260/280''' | | align="center" style="background:#f0f0f0;"|'''260/280''' | ||
| align="center" style="background:#f0f0f0;"|'''ng/µL''' | | align="center" style="background:#f0f0f0;"|'''ng/µL''' | ||
| align="center" style="background:#f0f0f0;"|'''ng/µL''' | |||
| align="center" style="background:#f0f0f0;"|'''average''' | |||
| align="center" style="background:#f0f0f0;"|'''ul for 400ng''' | | align="center" style="background:#f0f0f0;"|'''ul for 400ng''' | ||
| align="center" style="background:#f0f0f0;"|'''ul ann buff''' | | align="center" style="background:#f0f0f0;"|'''ul ann buff''' | ||
| align="center" style="background:#f0f0f0;"|'''ul H2O''' | | align="center" style="background:#f0f0f0;"|'''ul H2O''' | ||
|- | |- | ||
| Luc14|| | | Luc14||g029||0.073||0.039||1.889||76.357||73.096||74.73||5.35||1.50||8.15 | ||
|- | |- | ||
| Luc14|| | | Luc14||g030||0.043||0.023||1.889||43.492||43.386||43.44||9.21||1.50||4.29 | ||
|- | |- | ||
| Luc14|| | | Luc14||g033||0.084||0.045||1.837||80.736||83.56||82.15||4.87||1.50||8.63 | ||
|- | |- | ||
| Luc14|| | | Luc14||g036||0.082||0.044||1.88||82.825||82.088||82.46||4.85||1.50||8.65 | ||
|- | |- | ||
| Luc14||g040||0. | | Luc14||g040||0.065||0.036||1.794||63.731||64.87||64.30||6.22||1.50||7.28 | ||
|- | |- | ||
| Luc14||g041||0. | | Luc14||g041||0.055||0.029||1.888||53.011||54.684||53.85||7.43||1.50||6.07 | ||
|- | |- | ||
| Luc14||g042||0. | | Luc14||g042||0.045||0.024||1.885||47.205||45.157||46.18||8.66||1.50||4.84 | ||
|- | |- | ||
| Luc14||g043||0. | | Luc14||g043||0.044||0.023||1.88||45.143||43.898||44.52||8.98||1.50||4.52 | ||
|} | |} | ||
Latest revision as of 01:06, 27 September 2017
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08/27/2015Collected g034 transfections at 48 hours. Flow and gDNA extraction, three wells for g4+p and three wells for g4+d
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