Haynes Lab:Notebook/CRISPR Editing/2015/08/25
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08/25/2015Transfect gal4+puro and gal4+dox with g034, collect at 24, 48, and 72 hours Step 1: Making .5ug/10ul plasmid stocks
Step 2: make DNA + optimem mixes
Step 3: add plus reagent to DNA+ opti
step 4: Make optimem + lipo mastermix
Step 5: mix dna mix and lipo mix
Step 6: add lipo mix to cells
Transfected 2 wells for each siRNA and each cell type
Mix oligofectamine and optimem
Mix DNA and oligofectamine
While complexes form, prep cells
Add complexes to cells
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