Haynes Lab:Notebook/CRISPR Editing/2015/07/28: Difference between revisions
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Template master mixes | Template master mixes (Nanodrop reads, ng/uL) | ||
* Note: Donor DNA are column-cleaned PCR products; make dilutions in case the concentrations are too high | |||
* Multiplier = 3 rxns x 3 reps + 3 = 12 | * Multiplier = 3 rxns x 3 reps + 3 = 12 | ||
# CRISPR 1d | # CRISPR 1d (135.9) | ||
# CRISPR 2d | # CRISPR 2d (204.9) | ||
# CRISPR 3d | # CRISPR 3d (11.3) | ||
# CRISPR 4d | # CRISPR 4d (145.7) | ||
# donor Stop@72 | # donor Stop@72 & 1:10 dilution (120.6 & 17.8) | ||
# donor Stop@162 | # donor Stop@162 & 1:10 dilution (95.1 & 12.1) | ||
# donor Stop@255 | # donor Stop@255 & 1:10 dilution (83.2 & 10.2) | ||
# no template control (water) | # no template control (water) | ||
Revision as of 06:14, 29 July 2015
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||
07/28/2015
mCherry qPCR/melt pre-course test (at CSH)
Note: reference primers (GAPDH B2) are not here yet
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PCR - Bio-Rad CFX96
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