Haynes Lab:Notebook/CRISPR Editing/2015/07/28: Difference between revisions
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# CRISPR 3d | # CRISPR 3d | ||
# CRISPR 4d | # CRISPR 4d | ||
# donor Stop@72 | # donor Stop@72 (1:10 dilution) | ||
# donor Stop@162 | # donor Stop@162 (1:10 dilution) | ||
# donor Stop@255 | # donor Stop@255 (1:10 dilution) | ||
# no template control (water) | # no template control (water) | ||
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| Reagent || Vol. μL || (x12) | | Reagent || Vol. μL || (x12) | ||
|- | |- | ||
| DNA ( | | DNA (20 ng) || 3.0 || ## | ||
|- | |- | ||
| PCR H<sub>2</sub> | | PCR H<sub>2</sub>O || 4.5 || ## | ||
|- | |- | ||
| || | | || 4.5 || 54.0 | ||
|} | |} | ||
Revision as of 14:03, 28 July 2015
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07/28/2015
mCherry qPCR/melt pre-course test (at CSH)
Note: reference primers (GAPDH B2) are not here yet
PCR - Bio-Rad CFX96
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