Haynes Lab:Notebook/CRISPR Editing/2015/07/28: Difference between revisions
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Line 68: | Line 68: | ||
| DNA (10 ng) || 3.0 || 81.0 | | DNA (10 ng) || 3.0 || 81.0 | ||
|- | |- | ||
| | | PCR H<sub>2</sub>O2x SYBR (Roche) || 7.5 || 202.5 | ||
|- | |- | ||
| || 10.5 || 283.5 | | || 10.5 || 283.5 | ||
Line 75: | Line 75: | ||
PCR - Bio-Rad CFX96 | PCR - Bio-Rad CFX96 | ||
* 95°C, 3 min | * 95°C, 3 min | ||
* | * 40x [95°C, 10 sec / 57°C, 10 sec / 72°C, 10 sec (measure)] | ||
* 72°C, 30 sec | * 72°C, 30 sec | ||
* Melt: 70°C/ 5 sec -- +0.1°C (measure) --> 95°C | * Melt: 70°C/ 5 sec -- +0.1°C (measure) --> 95°C |
Revision as of 13:57, 28 July 2015
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07/28/2015
mCherry qPCR/melt pre-course test (at CSH)
Note: reference primers (GAPDH B2) are not here yet
PCR - Bio-Rad CFX96
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