Haynes Lab:Notebook/CRISPR Editing/2015/07/28: Difference between revisions
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PCR - Bio-Rad CFX96 | PCR - Bio-Rad CFX96 | ||
95°C, 3 min | * 95°C, 3 min | ||
30x [95°C, 10 sec / 57°C, 10 sec / 72°C, 10 sec (measure)] | * 30x [95°C, 10 sec / 57°C, 10 sec / 72°C, 10 sec (measure)] | ||
72°C, 30 sec | * 72°C, 30 sec | ||
Melt: 70°C -- +0.1°C | * Melt: 70°C/ 5 sec -- +0.1°C (measure) --> 95°C | ||
Revision as of 13:40, 28 July 2015
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||
07/28/2015
mCherry qPCR/melt pre-course test (at CSH)
Note: reference primers (GAPDH B2) are not here yet
PCR - Bio-Rad CFX96
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