Haynes Lab:Notebook/CRISPR Editing/2015/07/28: Difference between revisions
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* mCherry qPCR/melt pre-course test (at CSH) | |||
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'''mCherry qPCR/melt pre-course test (at CSH)''' | |||
* Templates: gDNA U-2 OS KAH154: CRISPR 1, 1d, 2, 2d, 3, 3d, 4, 4d | |||
* Primer pairs: | |||
** P147/148 (stop codons @72) = 148 bp | |||
** P143/144 (@162) = 100 bp | |||
** P145/146 (@255) = 100 bp | |||
* Templates & corresponding Primers: | |||
** CRISPR 1, P143/146 = 199 bp | |||
** CRISPR 1d, P143/146 = 199 bp | |||
Revision as of 11:54, 28 July 2015
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07/28/2015
mCherry qPCR/melt pre-course test (at CSH)
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