Haynes Lab:Notebook/CRISPR Editing/2015/07/28: Difference between revisions

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'''mCherry qPCR/melt pre-course test (at CSH)'''
'''mCherry qPCR/melt pre-course test (at CSH)'''
* Templates: gDNA U-2 OS KAH154: CRISPR 1, 1d, 2, 2d, 3, 3d, 4, 4d
* In 2014, a single gBlock matching all of mCherry
* Templates: gDNA U-2 OS KAH154: CRISPR 1d, 2d, 3d, 4d, PCR donor Stop@72, PCR donor Stop@162, PCR donor Stop@255
* Primer pairs:
* Primer pairs:
** P147/148 (stop codons @72) = 148 bp
** P147/148 (stop codons @72) = 148 bp
** P143/144 (@162) = 100 bp
** P143/144 (...@162) = 100 bp
** P145/146 (@255) = 100 bp
** P145/146 (...@255) = 100 bp
* Templates & corresponding Primers:
 
** CRISPR 1, P145/146 = 100 bp
** CRISPR 1d, P145/146 = 100 bp
** ''Note: CRISPR 1 INDELs of >9 bp may disrupt binding of P145''




Revision as of 12:19, 28 July 2015

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07/28/2015

  • mCherry qPCR/melt pre-course test (at CSH)


mCherry qPCR/melt pre-course test (at CSH)

  • In 2014, a single gBlock matching all of mCherry
  • Templates: gDNA U-2 OS KAH154: CRISPR 1d, 2d, 3d, 4d, PCR donor Stop@72, PCR donor Stop@162, PCR donor Stop@255
  • Primer pairs:
    • P147/148 (stop codons @72) = 148 bp
    • P143/144 (...@162) = 100 bp
    • P145/146 (...@255) = 100 bp




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