Haynes Lab:Notebook/CRISPR Editing/2015/07/03: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
Line 9: Line 9:
Finished gDNA extraction from Luc14s transfected with dilutions of g034
Finished gDNA extraction from Luc14s transfected with dilutions of g034
<br><br>
<br><br>
Collected 0.1 and 0.01ug g034 transfected gal4-eed cells with dox or puro for flow and gDNA extraction:
Collected 0.1 and 0.01ug g034 transfected Luc14s and gal4-eed cells with dox (6/30) for flow and gDNA extraction:
<br>
<br>
:aspirate media
:aspirate media

Revision as of 15:47, 5 July 2015

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

07/03/2015

Finished gDNA extraction from Luc14s transfected with dilutions of g034

Collected 0.1 and 0.01ug g034 transfected Luc14s and gal4-eed cells with dox (6/30) for flow and gDNA extraction:

aspirate media
add 400ul PBS, rock plate, aspirate PBS
add 200ul trypsin, let sit for a few minutes, tap plate to unstick cells
add 500ul media, pipette to collect cells, move to 1.5mL microcentrifuge tube
remove tubes from hood
spin in benchtop centrifuge at 0.2x1000RCF for 3 minutes
remove media with P1000
resuspend in 400ul cold PBS
filter 200ul for flow, use remaining 200ul for gDNA extraction



Retrieved from "https://openwetware.org/mediawiki/index.php?title=Haynes_Lab:Notebook/CRISPR_Editing/2015/07/03&oldid=891868"