Haynes Lab:Notebook/CRISPR Editing/2015/05/22: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Rene M Davis (talk | contribs) |
Rene M Davis (talk | contribs) |
||
Line 35: | Line 35: | ||
| align="center" style="background:#f0f0f0;"|'''rep''' | | align="center" style="background:#f0f0f0;"|'''rep''' | ||
| align="center" style="background:#f0f0f0;"|'''ng/µL''' | | align="center" style="background:#f0f0f0;"|'''ng/µL''' | ||
| align="center" style="background:#f0f0f0;"|'''dilute 1: | | align="center" style="background:#f0f0f0;"|'''dilute 1:20''' | ||
| align="center" style="background:#f0f0f0;"|'''ul dilution''' | | align="center" style="background:#f0f0f0;"|'''ul dilution''' | ||
| align="center" style="background:#f0f0f0;"|'''ul water''' | | align="center" style="background:#f0f0f0;"|'''ul water''' |
Revision as of 13:05, 22 May 2015
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
05/22/2015SYBR qPCR to determine plasmid counts for dilution experiment
Step 1: make two PCR tubes for each sample with the following volumes of water and gDNA for 2ng of gDNA per well
Step 2: make primer master mixes. Will have to add 3.5x to each triplicate gDNA tube so can calculate mastermixes from that
Step 3: mix gDNA and primer mixes
Step 4: pipette plate
|