Haynes Lab:Notebook/CRISPR Editing/2015/05/22: Difference between revisions
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Revision as of 11:43, 22 May 2015
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05/22/2015SYBR qPCR to determine plasmid counts for dilution experiment
Step 1: make two PCR tubes for each sample with the following volumes of water and gDNA for 2ng of gDNA per well
Step 2: make primer master mixes. Will have to add 3.5x to each triplicate gDNA tube so can calculate mastermixes from that
Step 3: mix gDNA and primer mixes
Step 4: pipette plate
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