Haynes Lab:Notebook/CRISPR Editing/2015/05/21: Difference between revisions
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==05/21/2015== | ==05/21/2015== | ||
Picked colonies from KAH228 in MV2 and KAH60 in pCvN transformation plates to miniprep tomorrow<br><br> | |||
Specd plasmid dilution nested PCR products | Specd plasmid dilution nested PCR products | ||
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Revision as of 14:17, 21 May 2015
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<html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
05/21/2015Picked colonies from KAH228 in MV2 and KAH60 in pCvN transformation plates to miniprep tomorrow
Annealed for surveyor, 20ul annealing reactions because of the lower concentrations
1ul surveyor, 1ul enhancer into tubes 1-8, 1ul water, 1ul enhancer into tube 9, 42degC for 1hour | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
