Haynes Lab:Notebook/CRISPR Editing/2015/05/21

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05/21/2015

Specd plasmid dilution nested PCR products

Cell type Plasmid ug plasmid ul lipo Rep 260 280 260/280 ng/µL
Gal4-EED g034 0.5 3 1 0.026 0.013 1.984 26.083
Gal4-EED g034 0.5 3 2 0.029 0.015 1.896 28.889
Gal4-EED g034 1 3 1 0.025 0.013 1.95 24.681
Gal4-EED g034 1 3 2 0.023 0.011 2.037 22.943
Gal4-EED g034 0.5 5 1 0.026 0.015 1.771 26.425
Gal4-EED g034 0.5 5 2 0.026 0.014 1.886 26.035
Gal4-EED g034 1 5 1 0.024 0.012 1.942 24.063
Gal4-EED g034 1 5 2 0.029 0.015 1.878 28.85

Annealed for surveyor, 20ul annealing reactions because of the lower concentrations

Cell type Plasmid ug plasmid ul lipo Rep ng/µL ul for 400ng ul anealing buff ul water
Gal4-EED g034 0.5 3 1 26.083 15.34 2.00 2.66
Gal4-EED g034 0.5 3 2 28.889 13.85 2.00 4.15
Gal4-EED g034 1 3 1 24.681 16.21 2.00 1.79
Gal4-EED g034 1 3 2 22.943 17.43 2.00 0.57
Gal4-EED g034 0.5 5 1 26.425 15.14 2.00 2.86
Gal4-EED g034 0.5 5 2 26.035 15.36 2.00 2.64
Gal4-EED g034 1 5 1 24.063 16.62 2.00 1.38
Gal4-EED g034 1 5 2 28.85 13.86 2.00 4.14


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