Haynes Lab:Notebook/CRISPR Editing/2015/05/20: Difference between revisions

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(Autocreate 2015/05/20 Entry for Haynes_Lab:Notebook/CRISPR_Editing)
 
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| colspan="2"|
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==03//2015==
==05/20/2015==
 
Tested 5 primer sets from Josh for internal controls for determining plasmid dilutions. Retested my pX330 primer sets. <br>
 
Primer master mixes 750ng/ul, used Luc14 g034 treated gDNA
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''gDNA ng/rxn'''
|-
| 100
|-
| 10
|-
| 5
|-
| 1
|-
| 0.5
|-
| 0.1
|-
| 0.05
|-
| 0.01
|-
| 0.005
|-
| 0.001
|-
| 0
|}


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Revision as of 11:55, 22 May 2015

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05/20/2015

Tested 5 primer sets from Josh for internal controls for determining plasmid dilutions. Retested my pX330 primer sets.
Primer master mixes 750ng/ul, used Luc14 g034 treated gDNA

gDNA ng/rxn
100
10
5
1
0.5
0.1
0.05
0.01
0.005
0.001
0


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