Haynes Lab:Notebook/CRISPR Editing/2015/05/06: Difference between revisions

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(Autocreate 2015/05/06 Entry for Haynes_Lab:Notebook/CRISPR_Editing)
 
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| colspan="2"|
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==03//2015==
==05/06/2015==
 
purified nested PCR of off-target gRNAs from Luc14
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Sample Read#'''
| align="center" style="background:#f0f0f0;"|'''260'''
| align="center" style="background:#f0f0f0;"|'''280'''
| align="center" style="background:#f0f0f0;"|'''260/280'''
| align="center" style="background:#f0f0f0;"|'''ng/µL'''
| align="center" style="background:#f0f0f0;"|'''ul for 400ng'''
|-
| g051||0.052||0.027||1.911||51.547||7.76
|-
| g052||0.058||0.031||1.85||58.161||6.88
|-
| g053||0.071||0.038||1.851||71.088||5.63
|-
| g054||0.065||0.035||1.855||65.16||6.14
|-
| g055||0.088||0.047||1.874||87.619||4.57
|-
| g056||0.061||0.035||1.737||61.227||6.53
|}





Revision as of 15:02, 6 May 2015

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05/06/2015

purified nested PCR of off-target gRNAs from Luc14

Sample Read# 260 280 260/280 ng/µL ul for 400ng
g051 0.052 0.027 1.911 51.547 7.76
g052 0.058 0.031 1.85 58.161 6.88
g053 0.071 0.038 1.851 71.088 5.63
g054 0.065 0.035 1.855 65.16 6.14
g055 0.088 0.047 1.874 87.619 4.57
g056 0.061 0.035 1.737 61.227 6.53