Haynes Lab:Notebook/CRISPR Editing/2015/05/02: Difference between revisions

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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
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|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}}
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==03//2015==
==05/02/2015==
 
Gal4-EED cells, +dox for 96 hours, -dox and transfected with either KAH228 or GFP, let grow for about 12 days, transfected with g025, g032, g046, or g048 on 4/29 at 6:30pm in triplicate.<br>
 
Collected cells in lysis buffer, heat for 18mins at 56deg C, put at -20
<br><br>
Luciferase assay<br>


{| {{table}}
| align="center" style="background:#f0f0f0;"|''''''
| align="center" style="background:#f0f0f0;"|'''avg'''
| align="center" style="background:#f0f0f0;"|'''std dev'''
|-
| Luc14||2.75||0.08
|-
| KAH228||1.02||0.06
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| GFP||0.76||0.01
|}
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__NOTOC__
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Latest revision as of 00:56, 27 September 2017

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05/02/2015

Gal4-EED cells, +dox for 96 hours, -dox and transfected with either KAH228 or GFP, let grow for about 12 days, transfected with g025, g032, g046, or g048 on 4/29 at 6:30pm in triplicate.
Collected cells in lysis buffer, heat for 18mins at 56deg C, put at -20

Luciferase assay

' avg std dev
Luc14 2.75 0.08
KAH228 1.02 0.06
GFP 0.76 0.01


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