Haynes Lab:Notebook/CRISPR Editing/2015/03/27

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03/27/2015

Ran on gel, purified, and set up digests of nested PCR products from yesterday for g034, unt and g048

Cell type Treatment rep F primer R primer 260 280 260/280 ng/µL ul for 200ng ul for 400ng ul water ul buffer ul enzyme
Luc14 g034 1 P163 P215 0.054 0.029 1.834 54.054 7.40 9.60 2 1
Luc14 g034 2 P163 P215 0.053 0.028 1.869 52.537 7.61 9.39 2 1
Luc14 g034 3 P163 P215 0.056 0.03 1.835 55.65 7.19 9.81 2 1
Gal4-EED +dox g034 1 P163 P215 0.055 0.031 1.8 54.901 7.29 9.71 2 1
Gal4-EED +dox g034 2 P163 P215 0.059 0.032 1.851 59.365 6.74 10.26 2 1
Gal4-EED +dox g034 3 P163 P215 0.052 0.027 1.882 51.558 7.76 9.24 2 1
Luc14 Unt 1 P163 P215 0.057 0.032 1.817 57.356 6.97 10.03 2 1
Luc14 Unt 2 P163 P215 0.063 0.034 1.872 63.42 6.31 10.69 2 1
Luc14 Unt 3 P163 P215 0.052 0.027 1.889 51.547 7.76 9.24 2 1
Gal4-EED +dox Unt 1 P163 P215 0.046 0.025 1.831 45.566 8.78 8.22 2 1
Gal4-EED +dox Unt 2 P163 P215 0.049 0.028 1.719 48.879 8.18 8.82 2 1
Gal4-EED +dox Unt 3 P163 P215 0.048 0.027 1.789 47.546 8.41 8.59 2 1
Luc14 g048 1 P213 P214 0.044 0.024 1.805 43.885 4.56 12.44 2 1
Luc14 g048 2 P213 P214 0.043 0.023 1.886 42.798 4.67 12.33 2 1
Luc14 g048 3 P213 P214 0.036 0.02 1.837 36.491 5.48 11.52 2 1
Gal4-EED +dox g048 1 P213 P214 0.033 0.019 1.772 33.381 5.99 11.01 2 1
Gal4-EED +dox g048 2 P213 P214 0.042 0.025 1.707 42.341 4.72 12.28 2 1
Gal4-EED +dox g048 3 P213 P214 0.036 0.021 1.735 36.042 5.55 11.45 2 1



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