Haynes Lab:Notebook/CRISPR Editing/2015/03/27: Difference between revisions

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(Autocreate 2015/03/27 Entry for Haynes_Lab:Notebook/CRISPR_Editing)
 
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==03//2015==
==03/27/2015==


Ran on gel, purified, and set up digests of nested PCR products from yesterday for g034, unt and g048
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Cell type'''
| align="center" style="background:#f0f0f0;"|'''Treatment'''
| align="center" style="background:#f0f0f0;"|'''rep'''
| align="center" style="background:#f0f0f0;"|'''F primer'''
| align="center" style="background:#f0f0f0;"|'''R primer'''
| align="center" style="background:#f0f0f0;"|'''260'''
| align="center" style="background:#f0f0f0;"|'''280'''
| align="center" style="background:#f0f0f0;"|'''260/280'''
| align="center" style="background:#f0f0f0;"|'''ng/µL'''
| align="center" style="background:#f0f0f0;"|'''ul for 200ng'''
| align="center" style="background:#f0f0f0;"|'''ul for 400ng'''
| align="center" style="background:#f0f0f0;"|'''ul water'''
| align="center" style="background:#f0f0f0;"|'''ul buffer'''
| align="center" style="background:#f0f0f0;"|'''ul enzyme'''
|-
| Luc14||g034||1||P163||P215||0.054||0.029||1.834||54.054||||7.40||9.60||2||1
|-
| Luc14||g034||2||P163||P215||0.053||0.028||1.869||52.537||||7.61||9.39||2||1
|-
| Luc14||g034||3||P163||P215||0.056||0.03||1.835||55.65||||7.19||9.81||2||1
|-
| Gal4-EED +dox||g034||1||P163||P215||0.055||0.031||1.8||54.901||||7.29||9.71||2||1
|-
| Gal4-EED +dox||g034||2||P163||P215||0.059||0.032||1.851||59.365||||6.74||10.26||2||1
|-
| Gal4-EED +dox||g034||3||P163||P215||0.052||0.027||1.882||51.558||||7.76||9.24||2||1
|-
| Luc14||Unt||1||P163||P215||0.057||0.032||1.817||57.356||||6.97||10.03||2||1
|-
| Luc14||Unt||2||P163||P215||0.063||0.034||1.872||63.42||||6.31||10.69||2||1
|-
| Luc14||Unt||3||P163||P215||0.052||0.027||1.889||51.547||||7.76||9.24||2||1
|-
| Gal4-EED +dox||Unt||1||P163||P215||0.046||0.025||1.831||45.566||||8.78||8.22||2||1
|-
| Gal4-EED +dox||Unt||2||P163||P215||0.049||0.028||1.719||48.879||||8.18||8.82||2||1
|-
| Gal4-EED +dox||Unt||3||P163||P215||0.048||0.027||1.789||47.546||||8.41||8.59||2||1
|-
| Luc14||g048||1||P213||P214||0.044||0.024||1.805||43.885||4.56||||12.44||2||1
|-
| Luc14||g048||2||P213||P214||0.043||0.023||1.886||42.798||4.67||||12.33||2||1
|-
| Luc14||g048||3||P213||P214||0.036||0.02||1.837||36.491||5.48||||11.52||2||1
|-
| Gal4-EED +dox||g048||1||P213||P214||0.033||0.019||1.772||33.381||5.99||||11.01||2||1
|-
| Gal4-EED +dox||g048||2||P213||P214||0.042||0.025||1.707||42.341||4.72||||12.28||2||1
|-
| Gal4-EED +dox||g048||3||P213||P214||0.036||0.021||1.735||36.042||5.55||||11.45||2||1
|}




Revision as of 09:30, 27 March 2015

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03/27/2015

Ran on gel, purified, and set up digests of nested PCR products from yesterday for g034, unt and g048

Cell type Treatment rep F primer R primer 260 280 260/280 ng/µL ul for 200ng ul for 400ng ul water ul buffer ul enzyme
Luc14 g034 1 P163 P215 0.054 0.029 1.834 54.054 7.40 9.60 2 1
Luc14 g034 2 P163 P215 0.053 0.028 1.869 52.537 7.61 9.39 2 1
Luc14 g034 3 P163 P215 0.056 0.03 1.835 55.65 7.19 9.81 2 1
Gal4-EED +dox g034 1 P163 P215 0.055 0.031 1.8 54.901 7.29 9.71 2 1
Gal4-EED +dox g034 2 P163 P215 0.059 0.032 1.851 59.365 6.74 10.26 2 1
Gal4-EED +dox g034 3 P163 P215 0.052 0.027 1.882 51.558 7.76 9.24 2 1
Luc14 Unt 1 P163 P215 0.057 0.032 1.817 57.356 6.97 10.03 2 1
Luc14 Unt 2 P163 P215 0.063 0.034 1.872 63.42 6.31 10.69 2 1
Luc14 Unt 3 P163 P215 0.052 0.027 1.889 51.547 7.76 9.24 2 1
Gal4-EED +dox Unt 1 P163 P215 0.046 0.025 1.831 45.566 8.78 8.22 2 1
Gal4-EED +dox Unt 2 P163 P215 0.049 0.028 1.719 48.879 8.18 8.82 2 1
Gal4-EED +dox Unt 3 P163 P215 0.048 0.027 1.789 47.546 8.41 8.59 2 1
Luc14 g048 1 P213 P214 0.044 0.024 1.805 43.885 4.56 12.44 2 1
Luc14 g048 2 P213 P214 0.043 0.023 1.886 42.798 4.67 12.33 2 1
Luc14 g048 3 P213 P214 0.036 0.02 1.837 36.491 5.48 11.52 2 1
Gal4-EED +dox g048 1 P213 P214 0.033 0.019 1.772 33.381 5.99 11.01 2 1
Gal4-EED +dox g048 2 P213 P214 0.042 0.025 1.707 42.341 4.72 12.28 2 1
Gal4-EED +dox g048 3 P213 P214 0.036 0.021 1.735 36.042 5.55 11.45 2 1



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