Haynes Lab:Notebook/CRISPR Editing/2015/03/24: Difference between revisions

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(Autocreate 2015/03/24 Entry for Haynes_Lab:Notebook/CRISPR_Editing)
 
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==03//2015==
==03/24/2015==
Ran on gel and did PCR purification kit for unt and g025 treated luc14 and gal4+dox cekks, nested PCR with P173/215. <br>
 
Surveyor hybridization protocol on 200ng in 15ul
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Cell type'''
| align="center" style="background:#f0f0f0;"|'''Treatment'''
| align="center" style="background:#f0f0f0;"|'''Rep'''
| align="center" style="background:#f0f0f0;"|'''260'''
| align="center" style="background:#f0f0f0;"|'''280'''
| align="center" style="background:#f0f0f0;"|'''260/280'''
| align="center" style="background:#f0f0f0;"|'''ng/µL'''
| align="center" style="background:#f0f0f0;"|'''ul for 200ng'''
| align="center" style="background:#f0f0f0;"|'''ul hyb buff'''
| align="center" style="background:#f0f0f0;"|'''ul water'''
|-
| Luc14||Unt||1||0.037||0.019||1.89||36.604||5.46||1.50||8.04
|-
| Luc14||Unt||2||0.025||0.014||1.745||25.137||7.96||1.50||5.54
|-
| Luc14||Unt||3||0.024||0.013||1.792||23.704||8.44||1.50||5.06
|-
| Gal4-EED+dox||Unt||1||0.027||0.015||1.79||26.773||7.47||1.50||6.03
|-
| Gal4-EED+dox||Unt||2||0.026||0.014||1.879||26.1||7.66||1.50||5.84
|-
| Gal4-EED+dox||Unt||3||0.022||0.012||1.874||21.554||9.28||1.50||4.22
|-
| Luc14||g025||1||0.023||0.012||1.897||23.003||8.69||1.50||4.81
|-
| Luc14||g025||2||0.024||0.013||1.763||23.653||8.46||1.50||5.04
|-
| Luc14||g025||3||0.023||0.014||1.649||22.935||8.72||1.50||4.78
|-
| Gal4-EED+dox||g025||1||0.023||0.013||1.76||23.111||8.65||1.50||4.85
|-
| Gal4-EED+dox||g025||2||0.018||0.01||1.765||17.939||11.15||1.50||2.35
|-
| Gal4-EED+dox||g025||3||0.026||0.016||1.59||26.08||7.67||1.50||5.83
|}
<br>
Cut with 1ul Surveyor enzyme and 1ul enhancer. Ran on BA.
<br><br>




Revision as of 12:06, 24 March 2015

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03/24/2015

Ran on gel and did PCR purification kit for unt and g025 treated luc14 and gal4+dox cekks, nested PCR with P173/215.

Surveyor hybridization protocol on 200ng in 15ul

Cell type Treatment Rep 260 280 260/280 ng/µL ul for 200ng ul hyb buff ul water
Luc14 Unt 1 0.037 0.019 1.89 36.604 5.46 1.50 8.04
Luc14 Unt 2 0.025 0.014 1.745 25.137 7.96 1.50 5.54
Luc14 Unt 3 0.024 0.013 1.792 23.704 8.44 1.50 5.06
Gal4-EED+dox Unt 1 0.027 0.015 1.79 26.773 7.47 1.50 6.03
Gal4-EED+dox Unt 2 0.026 0.014 1.879 26.1 7.66 1.50 5.84
Gal4-EED+dox Unt 3 0.022 0.012 1.874 21.554 9.28 1.50 4.22
Luc14 g025 1 0.023 0.012 1.897 23.003 8.69 1.50 4.81
Luc14 g025 2 0.024 0.013 1.763 23.653 8.46 1.50 5.04
Luc14 g025 3 0.023 0.014 1.649 22.935 8.72 1.50 4.78
Gal4-EED+dox g025 1 0.023 0.013 1.76 23.111 8.65 1.50 4.85
Gal4-EED+dox g025 2 0.018 0.01 1.765 17.939 11.15 1.50 2.35
Gal4-EED+dox g025 3 0.026 0.016 1.59 26.08 7.67 1.50 5.83


Cut with 1ul Surveyor enzyme and 1ul enhancer. Ran on BA.



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