Haynes Lab:Notebook/CRISPR Editing/2015/03/03: Difference between revisions

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Ran PCR products from P151/158 on gel. Only single product for each band.  
Ran PCR products from P151/158 on gel. Only single product for each band.  
<br><br>
<br><br>
Sequencing:<br>Need to purify. Will purify 25ul and elute into 25 ul.
Sequencing:<br>Need to purify. Will purify 25ul and elute into 35 ul.
 
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Cell type'''
| align="center" style="background:#f0f0f0;"|'''treatment'''
| align="center" style="background:#f0f0f0;"|'''rep'''
| align="center" style="background:#f0f0f0;"|'''260'''
| align="center" style="background:#f0f0f0;"|'''280'''
| align="center" style="background:#f0f0f0;"|'''260/280'''
| align="center" style="background:#f0f0f0;"|'''ng/µL'''
|-
| Luc14||Untreated||1||0.035||0.017||2.006||35.007
|-
| Luc14||g046||1||0.032||0.016||2.02||31.691
|-
| Luc14||Untreated||2||0.045||0.022||2.034||44.797
|-
| Luc14||Untreated||3||0.046||0.024||1.938||46.277
|-
| Gal4-EED +dox||Untreated||1||0.04||0.019||2.099||40.212
|-
| Gal4-EED +dox||Untreated||2||0.043||0.023||1.885||42.773
|-
| Gal4-EED +dox||Untreated||3||0.044||0.023||1.93||43.675
|-
| Luc14||g046||2||0.03||0.015||2.086||30.259
|-
| Luc14||g046||3||0.031||0.015||2.113||31.368
|-
| Gal4-EED +dox||g046||1||0.035||0.017||2.042||35.122
|-
| Gal4-EED +dox||g046||2||0.039||0.019||2.05||38.501
|-
| Gal4-EED +dox||g046||3||0.041||0.022||1.897||41.336
|-
| Luc14||Untreated||1||0.032||0.016||1.949||31.937
|-
| Luc14||g046||1||0.03||0.015||1.94||29.645
|}


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Revision as of 15:05, 3 March 2015

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02//2015

Checked concentrations on minipreps from last year of Lonza pMax GFP from Krishna. very low so JW transformed the original along with pMax GFP 6.2 and KAH60.

Sample Read# 260 280 260/280 ng/µL
pMax GFP 0.026 0.015 1.736 26.294
pMax GFP 0.023 0.013 1.766 23.175
pMax GFP 0.031 0.019 1.639 30.851



Ran PCR products from P151/158 on gel. Only single product for each band.

Sequencing:
Need to purify. Will purify 25ul and elute into 35 ul.

Cell type treatment rep 260 280 260/280 ng/µL
Luc14 Untreated 1 0.035 0.017 2.006 35.007
Luc14 g046 1 0.032 0.016 2.02 31.691
Luc14 Untreated 2 0.045 0.022 2.034 44.797
Luc14 Untreated 3 0.046 0.024 1.938 46.277
Gal4-EED +dox Untreated 1 0.04 0.019 2.099 40.212
Gal4-EED +dox Untreated 2 0.043 0.023 1.885 42.773
Gal4-EED +dox Untreated 3 0.044 0.023 1.93 43.675
Luc14 g046 2 0.03 0.015 2.086 30.259
Luc14 g046 3 0.031 0.015 2.113 31.368
Gal4-EED +dox g046 1 0.035 0.017 2.042 35.122
Gal4-EED +dox g046 2 0.039 0.019 2.05 38.501
Gal4-EED +dox g046 3 0.041 0.022 1.897 41.336
Luc14 Untreated 1 0.032 0.016 1.949 31.937
Luc14 g046 1 0.03 0.015 1.94 29.645


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