02//2015
Checked concentrations on minipreps from last year of Lonza pMax GFP from Krishna. very low so JW transformed the original along with pMax GFP 6.2 and KAH60.
Sample Read#
|
260
|
280
|
260/280
|
ng/µL
|
pMax GFP |
0.026 |
0.015 |
1.736 |
26.294
|
pMax GFP |
0.023 |
0.013 |
1.766 |
23.175
|
pMax GFP |
0.031 |
0.019 |
1.639 |
30.851
|
Ran PCR products from P151/158 on gel. Only single product for each band.
Sequencing: Need to purify. Will purify 25ul and elute into 35 ul.
Cell type
|
treatment
|
rep
|
260
|
280
|
260/280
|
ng/µL
|
Luc14 |
Untreated |
1 |
0.035 |
0.017 |
2.006 |
35.007
|
Luc14 |
g046 |
1 |
0.032 |
0.016 |
2.02 |
31.691
|
Luc14 |
Untreated |
2 |
0.045 |
0.022 |
2.034 |
44.797
|
Luc14 |
Untreated |
3 |
0.046 |
0.024 |
1.938 |
46.277
|
Gal4-EED +dox |
Untreated |
1 |
0.04 |
0.019 |
2.099 |
40.212
|
Gal4-EED +dox |
Untreated |
2 |
0.043 |
0.023 |
1.885 |
42.773
|
Gal4-EED +dox |
Untreated |
3 |
0.044 |
0.023 |
1.93 |
43.675
|
Luc14 |
g046 |
2 |
0.03 |
0.015 |
2.086 |
30.259
|
Luc14 |
g046 |
3 |
0.031 |
0.015 |
2.113 |
31.368
|
Gal4-EED +dox |
g046 |
1 |
0.035 |
0.017 |
2.042 |
35.122
|
Gal4-EED +dox |
g046 |
2 |
0.039 |
0.019 |
2.05 |
38.501
|
Gal4-EED +dox |
g046 |
3 |
0.041 |
0.022 |
1.897 |
41.336
|
Luc14 |
Untreated |
1 |
0.032 |
0.016 |
1.949 |
31.937
|
Luc14 |
g046 |
1 |
0.03 |
0.015 |
1.94 |
29.645
|
Label
|
Cell type
|
treatment
|
rep
|
PCR primer
|
1 |
Luc14 |
Untreated |
1 |
P176
|
2 |
Luc14 |
Untreated |
2 |
P176
|
3 |
Luc14 |
Untreated |
3 |
P176
|
4 |
Gal4-EED+dox |
Untreated |
1 |
P176
|
5 |
Gal4-EED+dox |
Untreated |
2 |
P176
|
6 |
Gal4-EED+dox |
Untreated |
3 |
P176
|
7 |
Luc14 |
g034 |
1 |
P176
|
8 |
Luc14 |
g034 |
2 |
P176
|
9 |
Luc14 |
g034 |
3 |
P176
|
10 |
Gal4-EED+dox |
g034 |
1 |
P176
|
11 |
Gal4-EED+dox |
g034 |
2 |
P176
|
12 |
Gal4-EED+dox |
g034 |
3 |
P176
|
13 |
Luc14 |
g046 |
1 |
P176
|
14 |
Luc14 |
g046 |
2 |
P176
|
15 |
Luc14 |
g046 |
3 |
P176
|
16 |
Gal4-EED+dox |
g046 |
1 |
P176
|
17 |
Gal4-EED+dox |
g046 |
2 |
P176
|
18 |
Gal4-EED+dox |
g046 |
3 |
P176
|
|