Haynes Lab:Notebook/CRISPR Editing/2015/03/02: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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==02 | ==03/02/2015== | ||
Induced Gal4-EED cells with dox. | |||
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I have P197/198 PCR products from Untreated, g046 treated, and g034 treated samples. I will dilute each of them 1:1000 fold and do nested PCR using primers P151 and P158. Annealing temp 68. | |||
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I will do PCR with P197/198 on g023, g045, and g048 samples. | |||
Latest revision as of 00:47, 27 September 2017
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03/02/2015Induced Gal4-EED cells with dox.
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