Haynes Lab:Notebook/CRISPR Editing/2015/02/07
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02/07/2015Estimated g023 P159/170 PCR product concentration based on gel from yesterday. Probably around 7ng/ul. Annealed 2x20ul of each product, didn't add anything or purify, according to the SURVEYOR protocol. Cut with 2ul SURVEYOR nuclease and 1ul of enhancer. Used 3ul of elution solution for -nuc samples. Incubated at 42 for one minute, then it went back to room temp because I set the new PCR machine incorrectly. Noticed after about 10-15 minutes, incubated for another hour.
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