Haynes Lab:Notebook/CRISPR Editing/2015/02/06: Difference between revisions

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(Autocreate 2015/02/06 Entry for Haynes_Lab:Notebook/CRISPR_Editing)
 
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| colspan="2"|
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==02//2015==
==02/06/2015==
Trying Surveyor on g023 with a shorter transcript. Still probably too long but I know these primers work and it's still like half of the transcript I was using before. First, PCR with phusion:


| {{table}}
| align="center" style="background:#f0f0f0;"|''''''
| align="center" style="background:#f0f0f0;"|'''1rxn'''
| align="center" style="background:#f0f0f0;"|'''6.2'''
|-
| H20||30.8||190.96
|-
| HF 5X MasterMix||10||62
|-
| dNTPs||0.2||1.24
|-
| FP P159||2.5||15.5
|-
| RP P170||2.5||15.5
|-
| Total||46||285.2
|}
{| {{table}}
| align="center" style="background:#f0f0f0;"|''''''
| align="center" style="background:#f0f0f0;"|'''1rxn'''
|-
| MM1||46
|-
| Template||2
|-
| DMSO||1.5
|-
| Phusion||0.5
|-
| Total||50
|}




Revision as of 12:56, 6 February 2015

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02/06/2015

Trying Surveyor on g023 with a shorter transcript. Still probably too long but I know these primers work and it's still like half of the transcript I was using before. First, PCR with phusion:

border="1" cellpadding="4" cellspacing="0" style="border:#c9c9c9 1px solid; margin: 1em 1em 1em 0; border-collapse: collapse;" ' 1rxn 6.2
H20 30.8 190.96
HF 5X MasterMix 10 62
dNTPs 0.2 1.24
FP P159 2.5 15.5
RP P170 2.5 15.5
Total 46 285.2
' 1rxn
MM1 46
Template 2
DMSO 1.5
Phusion 0.5
Total 50


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