Haynes Lab:Notebook/CRISPR Editing/2015/01/30: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
Rene M Davis (talk | contribs) |
Rene M Davis (talk | contribs) |
||
Line 12: | Line 12: | ||
We each prepped two tubes. Each contained 7μL of water with 2μL of plasmid template and 1μL of either DD122 primer or DD123 primer. The primers were obtained from Cameron. | We each prepped two tubes. Each contained 7μL of water with 2μL of plasmid template and 1μL of either DD122 primer or DD123 primer. The primers were obtained from Cameron. | ||
</font> | </font> | ||
<br> | <br><br> | ||
Picked 6 colonies from one of the g034 topo plates into 2mL LB+amp. Let grow overnight. | Picked 6 colonies from one of the g034 topo plates into 2mL LB+amp. Let grow overnight. | ||
<br><br> | <br><br> |
Revision as of 11:53, 31 January 2015
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
01/30/2015
Prepped KAH126 plasmids for sequencing from the mini preps we did on January 28, 2015 (Wed).
We each prepped two tubes. Each contained 7μL of water with 2μL of plasmid template and 1μL of either DD122 primer or DD123 primer. The primers were obtained from Cameron.
|