Haynes Lab:Notebook/CRISPR Editing/2015/01/23: Difference between revisions

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| align="center" style="background:#f0f0f0;"|'''ng/µL'''
| align="center" style="background:#f0f0f0;"|'''ng/µL'''
| align="center" style="background:#f0f0f0;"|'''ul for 1ug'''
| align="center" style="background:#f0f0f0;"|'''ul for 1ug'''
| align="center" style="background:#f0f0f0;"|'''ul H2O'''
| align="center" style="background:#f0f0f0;"|'''ul buffer'''
| align="center" style="background:#f0f0f0;"|'''ul PfoI'''
|-
|-
| Untreated||85.402||11.71
| Untreated||85.402||11.71||5.29||2||1
|-
|-
| g033||78.942||12.67
| g033||78.942||12.67||4.33||2||1
|-
|-
| g034||80.841||12.37
| g034||80.841||12.37||4.63||2||1
|}
|}


Revision as of 10:09, 23 January 2015

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01/23/2015

cutting 1μg of untreated, g033, and g034 treated Luc14 cells gDNA PCRd with P197/P198 and column purified samples from 14.12.06 experiment with PfoI at 37°C for 3 hours.

Sample Read# ng/µL ul for 1ug ul H2O ul buffer ul PfoI
Untreated 85.402 11.71 5.29 2 1
g033 78.942 12.67 4.33 2 1
g034 80.841 12.37 4.63 2 1


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