Haynes Lab:Notebook/CRISPR Editing/2015/01/20: Difference between revisions

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Prepared plasmids for transfection:
Prepared plasmids for transfection:
{| {{table}}
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''plasmid'''
| align="center" style="background:#f0f0f0;"|'''Sample Read#'''
| align="center" style="background:#f0f0f0;"|'''concentration'''
| align="center" style="background:#f0f0f0;"|'''ng/µL'''
| align="center" style="background:#f0f0f0;"|'''2ug x 6.5'''
| align="center" style="background:#f0f0f0;"|'''ul for 12ug'''
| align="center" style="background:#f0f0f0;"|'''ul H2O for 6.5'''
| align="center" style="background:#f0f0f0;"|'''ul H2O'''
|-
|-
| g023||391||33.25||31.75
| g023||391||30.69||29.31
|-
|-
| g034||302||43.05||21.95
| g025||235.95||50.86||9.14
|-
|-
| g044||403||32.26||32.74
| g031||288.187||41.64||18.36
|-
|-
| g045||334||38.92||26.08
| g032||452.739||26.51||33.49
|-
|-
| g046||310||41.94||23.06
| g034||302||39.74||20.26
|-
| g044||403||29.78||30.22
|-
| g045||334||35.93||24.07
|-
| g046||310||38.71||21.29
|-
| g048||345.075||34.78||25.22
|}
|}


Revision as of 15:44, 20 January 2015

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01/20/2015

induced gal4-eed cells were only at about 60-70% confluent so i split all the cells between the two 50mL conicals.


Prepared plasmids for transfection:

Sample Read# ng/µL ul for 12ug ul H2O
g023 391 30.69 29.31
g025 235.95 50.86 9.14
g031 288.187 41.64 18.36
g032 452.739 26.51 33.49
g034 302 39.74 20.26
g044 403 29.78 30.22
g045 334 35.93 24.07
g046 310 38.71 21.29
g048 345.075 34.78 25.22


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