Haynes Lab:Notebook/CRISPR Editing/2014/12/17: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(Autocreate 2014/12/17 Entry for Haynes_Lab:Notebook/CRISPR_Editing)
 
Line 6: Line 6:
| colspan="2"|
| colspan="2"|
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==07//2014==
==12/17/2014==
 
Gradient PCR of g029 treated Luc14 gDNA. 6 temperatures, 50ul reactions.<br>
 
:53°C
:55°C
:57°C
:59°C
:61°C
:63°C
Ran gel, only saw additional bands at 53°C, didn't see any drop off in yield. <br>
Set up 50ul PCR reactions for g023, g025, g030, untreated luc14 gDNA. annealing temp 63.


<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->

Revision as of 11:40, 18 December 2014

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

12/17/2014

Gradient PCR of g029 treated Luc14 gDNA. 6 temperatures, 50ul reactions.

53°C
55°C
57°C
59°C
61°C
63°C

Ran gel, only saw additional bands at 53°C, didn't see any drop off in yield.
Set up 50ul PCR reactions for g023, g025, g030, untreated luc14 gDNA. annealing temp 63.


Retrieved from "https://openwetware.org/mediawiki/index.php?title=Haynes_Lab:Notebook/CRISPR_Editing/2014/12/17&oldid=848497"