Haynes Lab:Notebook/CRISPR Editing/2014/10/29

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10/29/2014

specs for minipreps for gRNAs in pX330 23, 25, 31-38

Sample Read# 260 280 260/280 ng/µL
g023 0.14 0.074 1.897 140.269
g025 0.166 0.088 1.9 166.412
g031 0.158 0.083 1.904 157.698
g032 0.136 0.072 1.885 135.981
g033 0.008 0.004 1.945 8.34
0.009 0.004 2.044 8.645
g034 0.112 0.059 1.901 111.632
g035 0.113 0.06 1.892 113.472
g036 0.147 0.079 1.864 146.554
g037 0.015 0.008 1.866 14.866
0.017 0.009 1.859 16.959
g038 0.084 0.044 1.894 84.224
0.094 0.049 1.911 94.206

Will repick g033, g037, and g038 to grow up tonight, miniprep tomorrow morning.


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