Haynes Lab:Notebook/CRISPR Editing/2014/10/27: Difference between revisions
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::Cycle the previous two steps for 6 cycles (total run time 1h) | ::Cycle the previous two steps for 6 cycles (total run time 1h) | ||
:4°C hold until ready to proceed | :4°C hold until ready to proceed | ||
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Long transformation, used plates made by cameron and my cells. | |||
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Revision as of 10:24, 30 October 2014
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10/27/2014Phosphorylated and annealed gRNAs 23, 25, 31-38 for cloning into pX330.
Anneal in a thermocycler using the following parameters:
Incubate the ligation reaction in a thermocycler:
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