Haynes Lab:Notebook/CRISPR Editing/2014/10/22: Difference between revisions

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==10/22/2014==
==10/22/2014==
Sequencing
{| {{table}}
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Seq sample name'''
| align="center" style="background:#f0f0f0;"|'''Seq sample name'''

Revision as of 10:42, 18 November 2014

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10/22/2014

Sequencing

Seq sample name Template Primer Primer name F/R Distance from gRNA
1 Luc14 gRNA 20 Srvyr PCR P197 SRVYR Luc F2 F 390
2 Luc14 gRNA 20 Srvyr PCR P160 gRNA 029 ES P R R 238
3 Gal4-EED +dox 1 gRNA 20 Srvyr PCR P197 SRVYR Luc F2 F 390
4 Gal4-EED +dox 2 gRNA 20 Srvyr PCR P160 gRNA 029 ES P R R 238
5 Gal4-EED +dox 1 gRNA 20 Srvyr PCR P197 SRVYR Luc F2 F 390
6 Gal4-EED +dox 2 gRNA 20 Srvyr PCR P160 gRNA 029 ES P R R 238
7 Luc14 gRNA 21 Srvyr PCR P197 SRVYR Luc F2 F 259
8 Luc14 gRNA 22 Srvyr PCR P197 SRVYR Luc F2 F 341
9 Luc14 gRNA 22 Srvyr PCR P160 gRNA 029 ES P R R 284
10 Luc14 gRNA 27 Srvyr PCR P169 gRNA 026 US P F F 172
11 Luc14 gRNA 27 Srvyr PCR P198 SRVYR Luc R R 247
12 Luc14 gRNA 29 Srvyr PCR P149 gRNA 020 ES P F F 238
13 Luc14 gRNA 29 Srvyr PCR P176 gRNA 030 US P R R 195
14 Gal4-EED +dox 1 gRNA 29 Srvyr PCR P149 gRNA 020 ES P F F 238
15 Gal4-EED +dox 2 gRNA 29 Srvyr PCR P176 gRNA 030 US P R R 195
16 Gal4-EED +dox 1 gRNA 29 Srvyr PCR P149 gRNA 020 ES P F F 238
17 Gal4-EED +dox 2 gRNA 29 Srvyr PCR P176 gRNA 030 US P R R 195
18 Luc14 Srvyr PCR P197 SRVYR Luc F2 F
19 Luc14 Srvyr PCR P160 gRNA 029 ES P R R
20 Luc14 Srvyr PCR P169 gRNA 026 US P F F
21 Luc14 Srvyr PCR P198 SRVYR Luc R R
22 Luc14 Srvyr PCR P149 gRNA 020 ES P F F
23 Luc14 Srvyr PCR P176 gRNA 030 US P R R



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