Haynes Lab:Notebook/CRISPR Editing/2014/09/25: Difference between revisions
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Set up nested PCR with P199 and P200 of gRNA 20 samples and gRNA 29 samples and Luc14 genomic DNA (7 50ul reactions in triplicate to get enough) | Set up nested PCR with P199 and P200 of gRNA 20 samples and gRNA 29 samples and Luc14 genomic DNA (7 50ul reactions in triplicate to get enough)<br> | ||
Used 1ul of each template at 100-200ng/ul concentration for gRNA 20 samples. Used 1ul of 1:100 dilution of gRNA 29 samples and Luc14 gRNA control. | |||
Revision as of 10:52, 25 September 2014
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09/25/2014Purified PCR product for Surveyor, P197,P198 forgot to save some unpurified on a gel
Set up nested PCR with P199 and P200 of gRNA 20 samples and gRNA 29 samples and Luc14 genomic DNA (7 50ul reactions in triplicate to get enough)
Annealing protocol on wang lab machine
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