Haynes Lab:Notebook/CRISPR Editing/2014/09/25: Difference between revisions
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Set up nested PCR with P199 and P200 of gRNA 20 samples and gRNA 29 samples and Luc14 genomic DNA (7 50ul reactions in triplicate to get enough) | Set up nested PCR with P199 and P200 of gRNA 20 samples and gRNA 29 samples and Luc14 genomic DNA (7 50ul reactions in triplicate to get enough) | ||
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| Add 145ul to each first tube for each set of reactions | | Add 145ul to each first tube for each set of reactions | ||
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| add the following to each first tube | | add the following to each first tube||1rxn||3.053 rxns | ||
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| template||1||3.053 | | template||1||3.053 | ||
Revision as of 09:30, 25 September 2014
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09/25/2014Purified PCR product for Surveyor, P197,P198 forgot to save some unpurified on a gel
Set up nested PCR with P199 and P200 of gRNA 20 samples and gRNA 29 samples and Luc14 genomic DNA (7 50ul reactions in triplicate to get enough)
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