Haynes Lab:Notebook/CRISPR Editing/2014/09/23: Difference between revisions

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| Luc14 gRNA 29||0.159||0.086||1.858||159.485||2.51
| Luc14 gRNA 29||0.159||0.086||1.858||159.485||2.51
|}
|}
Set up annealing reaction, 10ul total
{| {{table}}
| align="center" style="background:#f0f0f0;"|''''''
| align="center" style="background:#f0f0f0;"|'''ul for 400ng'''
| align="center" style="background:#f0f0f0;"|'''ul elution solution'''
| align="center" style="background:#f0f0f0;"|'''ul water'''
| align="center" style="background:#f0f0f0;"|'''ul 10x annealing buffer'''
|-
| gRNA 20||2.85||0.15||6||1
|-
| gRNA 21||2.28||0.72||6||1
|-
| gRNA 22||2.02||0.98||6||1
|-
| gRNA 27||3.05||-0.05||6||1
|-
| gRNA 29||2.51||0.49||6||1
|}
<br>


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Revision as of 15:09, 23 September 2014

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09/23/2014

Running 2ul of each PCR rxn on a gel, with 3ul of water and 1ul of 6x loading dye.
figuring out volumes of wells so I can know exactly what volume to use for each annealing reaction.
purifying and concentrating all successful PCR rxns using column. should also figure out if 50x loading dye works.

Sample Read# 260 280 260/280 ng/µL ul for 400ng
Luc14 gRNA 20 0.141 0.075 1.864 140.539 2.85
Luc14 gRNA 21 0.176 0.098 1.788 175.643 2.28
Luc14 gRNA 22 0.198 0.106 1.869 197.974 2.02
Luc14 gRNA 27 0.131 0.07 1.868 131.014 3.05
Luc14 gRNA 29 0.159 0.086 1.858 159.485 2.51

Set up annealing reaction, 10ul total

' ul for 400ng ul elution solution ul water ul 10x annealing buffer
gRNA 20 2.85 0.15 6 1
gRNA 21 2.28 0.72 6 1
gRNA 22 2.02 0.98 6 1
gRNA 27 3.05 -0.05 6 1
gRNA 29 2.51 0.49 6 1



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