Haynes Lab:Notebook/CRISPR Editing/2014/09/23: Difference between revisions
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Rene M Davis (talk | contribs) |
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| Luc14 gRNA 29||0.159||0.086||1.858||159.485||2.51 | | Luc14 gRNA 29||0.159||0.086||1.858||159.485||2.51 | ||
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Set up annealing reaction, 10ul total | |||
{| {{table}} | |||
| align="center" style="background:#f0f0f0;"|'''''' | |||
| align="center" style="background:#f0f0f0;"|'''ul for 400ng''' | |||
| align="center" style="background:#f0f0f0;"|'''ul elution solution''' | |||
| align="center" style="background:#f0f0f0;"|'''ul water''' | |||
| align="center" style="background:#f0f0f0;"|'''ul 10x annealing buffer''' | |||
|- | |||
| gRNA 20||2.85||0.15||6||1 | |||
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| gRNA 21||2.28||0.72||6||1 | |||
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| gRNA 22||2.02||0.98||6||1 | |||
|- | |||
| gRNA 27||3.05||-0.05||6||1 | |||
|- | |||
| gRNA 29||2.51||0.49||6||1 | |||
|} | |||
<br> | |||
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Revision as of 15:09, 23 September 2014
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09/23/2014Running 2ul of each PCR rxn on a gel, with 3ul of water and 1ul of 6x loading dye.
Set up annealing reaction, 10ul total
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