Haynes Lab:Notebook/CRISPR Editing/2014/09/23: Difference between revisions

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purifying and concentrating all successful PCR rxns using column. should also figure out if 50x loading dye works.
purifying and concentrating all successful PCR rxns using column. should also figure out if 50x loading dye works.


{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Sample Read#'''
| align="center" style="background:#f0f0f0;"|'''260'''
| align="center" style="background:#f0f0f0;"|'''280'''
| align="center" style="background:#f0f0f0;"|'''260/280'''
| align="center" style="background:#f0f0f0;"|'''ng/µL'''
| align="center" style="background:#f0f0f0;"|'''ul for 400ng'''
|-
| Luc14 gRNA 20||0.141||0.075||1.864||140.539||2.846185045
|-
| Luc14 gRNA 21||0.176||0.098||1.788||175.643||2.277346663
|-
| Luc14 gRNA 22||0.198||0.106||1.869||197.974||2.020467334
|-
| Luc14 gRNA 27||0.131||0.07||1.868||131.014||3.053108828
|-
| Luc14 gRNA 29||0.159||0.086||1.858||159.485||2.50807286
|}


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Revision as of 13:58, 23 September 2014

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09/23/2014

Running 2ul of each PCR rxn on a gel, with 3ul of water and 1ul of 6x loading dye.
figuring out volumes of wells so I can know exactly what volume to use for each annealing reaction.
purifying and concentrating all successful PCR rxns using column. should also figure out if 50x loading dye works.

Sample Read# 260 280 260/280 ng/µL ul for 400ng
Luc14 gRNA 20 0.141 0.075 1.864 140.539 2.846185045
Luc14 gRNA 21 0.176 0.098 1.788 175.643 2.277346663
Luc14 gRNA 22 0.198 0.106 1.869 197.974 2.020467334
Luc14 gRNA 27 0.131 0.07 1.868 131.014 3.053108828
Luc14 gRNA 29 0.159 0.086 1.858 159.485 2.50807286


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