Haynes Lab:Notebook/CRISPR Editing/2014/09/23: Difference between revisions
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Added 1ul of enhancer and 1ul of nuclease to each reaction, incubate at 42 for 1 hour. | |||
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Ran gel 2ul of ladder, 1ul of each purified PCR reaction (lost a little of gRNA 22), and gRNA 27 unpurified reaction 2 that looked like it didn't work at all. | |||
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uncut controls of each gRNA purified pcr of the same volumes as annealing reactions. | |||
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Latest revision as of 00:19, 27 September 2017
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09/23/2014Running 2ul of each PCR rxn on a gel, with 3ul of water and 1ul of 6x loading dye.
Set up annealing reaction, 10ul total
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