Haynes Lab:Notebook/CRISPR Editing/2014/09/22

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09/22/2014

Cut the annealed Luc14 reactions, but not gRNA 21 because that one didn't amplify. added 40ul of annealed reaction to a new PCR tube, added 1ul of enhancer and 2ul of Surveyor nuclease. Incubated at 42°C for one hour, kept on ice after. Forgot to add stop solution. ran gel after about an hour or so on ice.
Ran gel:



Added 100ul of elution butffer (Sigma elution solution from genelute miniprep kit) to each stock of gDNA for each sample. Then did the dilutions from before but into 20ul of elution buffer.



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